A study was conducted to assess the study to assess the effectiveness of structured teaching programme on knowledge regarding awareness of HIV/AIDS among antenatal mothers in selected community area of Udaipur district The objectives were to as To assess the pre- test level of knowledge regarding HIV/AIDS among antenatal mother, To assess the post- test knowledge regarding HIV/AIDS among antenatal mother, To assess the effectiveness of structured teaching programme on knowledge regarding awareness of HIV/AIDS among antenatal mothers, To find out association between post -test level of knowledge with their selected socio demographic variables. the researcher also found that knowledge regarding awareness of HIV/AIDS among antenatal mothers. India has also the third largest number of people living with HIV/AIDS. The participation of antenatal mothers in the process and accessibility to prevention of mother to child transmission (PMTCT) information can prevent serious implication. Acquired immunodeficiency syndrome epidemic is the greatest challenge to human kind in the 21st century. Mother to child transmission (MTCT) of human immunodeficiency virus (HIV) infection is the transmission of the virus from HIV infected mother to child during pregnancy, labor, delivery or breastfeeding.