Coronavirus Disease is a pandemic that requires people worldwide to take quick precautions to avoid the infection. Nurses are considered the backbone of the healthcare system, especially during this Covid-19 pandemic. They make up the largest percentage of the workforce in any healthcare facility worldwide. In Malaysia, men and women have become increasingly engaged in healthcare. This is supported by the growth of nursing schools that educate Malaysian men and women to become the country's primary and top health professionals. This study aimed to identify the knowledge, attitude and practice of infection control measures among the Registered Nurses in one of the Specialist hospitals. The research design chosen in this study is a descriptive quantitative design. It will include a descriptive analysis of key demographics, variables, reliability analysis, Cronbach’s Alpha correlation of variables, Kruskal- Wallis, Mann- Whitney, Pearson Correlation and hypotheses testing. The method used for data collection is a self-administered questionnaire with 37 questions of Yes or No and a Likert Scale, which was distributed to 169 nurses The researcher will use the online survey using google Forms due to the pandemic situation. The researcher also will utilize several strategies to reach the respondents via Whats App messages. The population of this study is all the RN working at one of the private hospitals in Klang Valley. There was approximately 314 RN working in the hospital. According to Krejcie and Morgan's Table (1970), the total number sample size for the total population of 314 RN is 169 samples. Of 152 respondents, 151 (99.3%) had a good knowledge level, and only 1 (0.7%) had a moderate knowledge level on Covid-19. For the level of attitude, 152 (100%) of the respondents had a good attitude towards Covid-19. For the level of practice, all the respondents, 152 (100%), had good care practice for Covid-19 patients.