Background: Iron deficiency is the most prevalent nutritional deficiency all over the world. Globally, anemia affects 1.62 billion people (24.8%), among which 56 million (41.8%) are pregnant women. It is a major public health problem particularly among poorer segments of the population in developing countries where 95% of the world anemic pregnant women are residing. Objective: To assess the knowledge and practices regarding prevention of iron deficiency anemia among primi gravida women attending antenatal OPD. Methods: For the present study, Quantitative research approach was used. Descriptive research design was used to collect data from 100 primi gravida women. The research setting was OPD of SMGS hospital, GMC Jammu. The sample consisted of 100 primi gravida women. Purposive sampling technique was used to select the sample. Socio-demographic profile, a self structured questionnaire and a checklist was used to collect data. Results: The results of the study revealed that that maximum of the primi gravida women 57(57%) were having average knowledge followed by 31(31%) were having poor knowledge and only 12(12%) were having good knowledge regarding prevention of iron deficiency anaemia. In regard to practices, the study showed that maximum of the primi gravida women 57(57%) were having poor practices followed by 43(43%) were having good practices regarding prevention of iron deficiency anaemia. Conclusion: The study concluded that there is need to strengthening health education on anemia prevention during ANC follow up and preparations of brochureswhich describes symptoms, risks factors and ways of anemia preventions and distribute to every primi gravida mother during their visit to ANC clinic.